Monoclonal Anti-v-Src antibody produced in mouse

MFCD00239655

NA.44

Src family kinases are a group of structurally related non-receptor protein tyrosine kinases that can lead to a variety of cellular responses including differentiation and proliferation. All the members have an amino terminal that has the kinase function and the C-terminal that regulates the kinase function. The amino terminal has Src homology 4 domain (SH4), containing a myristylation signal sequence essential for membrane localization; conserved SH3 and SH2 domains, which mediate protein-protein interactions, separated from the SH4 by a unique region; a catalytic kinase domain. The major difference between v-Src and c-Src is the structure of the C terminal. Src is involved in the regulation of many cellular functions such as migration, adhesion, gene transcription, cell cycle, apoptosis and differentiation. Few signalling pathways through which Src regulates these processes are PI3K, STAT3, FAK and paxillin. Src overexpression is observed in human cancers.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.

Src protein (p60^src).

The recommended anti-v-Src antibody concentration is 2-5 μg/mL for immunoblotting using a COLO201 cell extract and chemiluminescence detection. The antibody is suitable for immunoprecipitation.

Lyophilized with 100 μg bovine serum albumin.

p60^src may appear as a doublet due to phosphorylation.

200

mouse

unconjugated

purified immunoglobulin

primary antibodies

327, monoclonal

lyophilized powder

antigen ~56 kDa

mouse, human, rat

immunoprecipitation (IP): suitable (suitable; may appear as doublet due to phosphorylation)
western blot: 2-5 μg/mL using COLO201 cell extract

IgG1κ

P12931

wet ice

2-8℃

human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)