VRB (Violet Red Bile Lactose) agar

ISO

200

FDA

medium granules (dehydrated (DCM))

GranuCult^®

microbiological culture: suitable

7.3-7.5 (37 ℃, 40 g/L in H₂O, after autoclaving)

39.5 g/L

670 kg/m³

food and beverages
microbiology

15-25℃

coliforms

Replaces: 1014065007

Appearance (clearness): clear
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 50 %
Colony colour (Escherichia coli ATCC 8739 (WDCM 00012)): purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 50 %
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Enterobacter cloacae ATCC 13047 (WDCM 00083)):
Colony count (Enterobacter cloacae ATCC 13047 (WDCM 00083)):
Recovery on test medium (Enterobacter cloacae ATCC 13047 (WDCM 00083)): ≥ 50 %
Colony colour (Enterobacter cloacae ATCC 13047 (WDCM 00083)): purplish-red colonies with or without precipitate halo
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Colony colour (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): colourless to beige colonies
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Incubation: 24 ± 2 hours at 30 ± 1 °C
Reference media: Tryptic Soy Agar
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 3