APN-COCI

C₁₀H₄ClNO

189.60

329768747

APN-COCl is a bifunctional reagent appropriate for the introduction of APN function.

The coupling between an amine and APN-COCl should be performed in dry organic solvents (e.g. THF) in the presence of a base (e.g. TEA). The resulting conjugate can be readily coupled with thiol-containing biomolecules using the following protocol:

• Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.

If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

powder

100

reagent type: cross-linking reagent

2-8℃

N#CC#CC1=CC=C(C(Cl)=O)C=C1

1S/C10H4ClNO/c11-10(13)9-5-3-8(4-6-9)2-1-7-12/h3-6H

OWNJCJKKKZXTIX-UHFFFAOYSA-N

50 mg in glass bottle

GHS05,GHS07

Danger

H302 - H314

P260 - P270 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Corr. 1B

8A - Combustible, corrosive hazardous materials

WGK 3