Anti-TAF4 antibody produced in rabbit

HPA008599    Human Protein Atlas characterization data

TAF4 (TATA-box binding protein associated factor 4) is a component of transcription factor complex TFIID, which forms an essential part of the transcriptional machinery. TFIID contains TATA-binding protein (TBP) and 14 TAFs. It contains two copies of TAF4. The N-terminal of TAF4 contains a conserved glutamine rich domain. Its core is composed of co-activator TAF4-homology ETO-TAFH/CRI/NHR1 (TAFH) domain, and its C-terminal contains a histone-like (CRII) domain.

Transcription initiation factor TFIID subunit 4 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

TAF4 (TATA-box binding protein associated factor 4) is responsible for maintaining the structural stability of the TFIID complex. It mediates interactions with CREB (cAMP response element-binding) and Sp1 (specificity protein) proteins, and as it is a part of the Wnt signaling, it plays a role in cell fate determination and differentiation and development. Its controlled degradation is linked to proper male germ-cell differentiation, and TAF4b is essential for the development of oocytes and spermatogenesis. It interacts with activation function-2 (AF-2), and induces the transcription of retinoic acid, vitamin D3 and thyroid hormone receptors. It is also involved in the regulation of neurongeneis and neuronal differentiation, where it interacts with TP53 and noncanonical Wnt signaling.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST70914.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

VLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCK

O00268

wet ice

−20℃

human ... TAF4(6874)

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10 - Combustible liquids

WGK 1

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)