Anti-Chicken Egg Albumin (Ovalbumin) antibody, Mouse monoclonal

NA.46

Anti-Chicken egg albumin (ovalbumin) antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma OVA-14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken egg albumin. The product reacts with turkey egg albumin, but not with serum albumin of the following species: chicken, turkey, human, bovine, pig, donkey, goat, sheep, horse, dog, cat, guinea pig, rabbit, rat, mouse, and pigeon.
Ovalbumin (323-339) is the most abundant egg white protein surrounded by B-cell epitopes, which are bound by specific IgE antibodies. In addition, it also contains CD4⁺ T cell epitopes. Ovalbumin is a monomeric protein with a molecular mass of 45000Da.

purified Chicken Egg Albumin

Ovalbumin (OVA) peptide 323-339 mediates 25–35% of T-cell response to intact OVA in isolated BALB/c mouse. OVA 323-339 has been widely used to determine the characteristics of class II major histocompatibility complex (MHC)-peptide binding and T-cell activation.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

200

mouse

purified immunoglobulin

primary antibodies

OVA-14, monoclonal

buffered aqueous solution

antigen 45 kDa

turkey, chicken

~1 mg/mL

competitive inhibition ELISA: suitable
dot blot: suitable
immunoblotting: suitable
indirect ELISA: 0.1-0.2 μg/mL using 10 μg/mL Albumin from chicken egg white (A5503) for coating.

IgG1

dry ice

−20℃

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids