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AFRC 84C6
产品性质
biological source【生物来源】 | Horse x Mouse/Horse Hybridoma |
ImmunoDon | Horse sub-mandibular lymph node |
Myeloma【骨髓瘤】 | AFRC 17D1 |
Reactivity【反应性】 | Anti-Haemagglutinin, influenza |
technique(s) | cell culture | mammalian: suitable |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Horse sub-mandibular lymph node x AFRC 17D1 |
Cell Line Description【细胞系描述】 | The heterohybridoma AFRC 84C6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84C6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825). |
Immunogen【免疫原】 | Influenza A equine 2 virus |
Application【应用】 | Haemagglutinin detection The isotype of the monoclonal antibody produced by this cell line is horse IgG. |
Culture Medium【培养基】 | RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS) |
Subculture Routine【传代培养常规】 | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃ |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |