Abberior^®CAGE 590, NHS-ester

NA.32

Abberior CAGE 590 is a masked dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye. The dye opens up the red fluorescent regime for CAGE dyes. It can most effectively be excited from 560 to 600nm.

Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyse molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.

Key Features

• First CAGE dye in the red fluorescent range
• Ideal for PALM, STORM, GSDIM
• Well suited as 2nd STED color (after uncaging)

Absorption Maximum, λmax: 262, 325, 351 nm (non-activated, PBS, pH 7.4)
595 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75,000 M^-1cm^-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 615 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 685 −715 nm
Fluorescence Quantum Yield, η: 0.80 (after photoactivation, PBS, pH 7.47)

Abberior^® CAGE 590 anti-rabbit antibody has been used as a secondary antibody for spectral-FPALM (fluorescence photo-activation localization microscopy) imaging of NIH-3T3 cells.

degree of coupling: report results

For more information on Abberior^® Dyes, please visit our overview.

abberior is a registered trademark of Abberior GmbH

DMF: 0.25 mg/mL, clear

λ_ex 590 nm; λ_em 600-620 nm in PBS, pH 7

−20℃

Designed and tested for fluorescent super-resolution microscopy

13 - Non Combustible Solids

WGK 3