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产品性质
| biological source【生物来源】 | fish ovary |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 60 |
| morphology【形态学】 | Fibroblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Trout Rainbow gonad tissue |
| Cell Line Description【细胞系描述】 | From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24℃. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE). |
| Application【应用】 | Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE) |
| Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Passage using 0.25% trypsin below 24℃. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24℃. Fish cell lines detach easily during transit if the culture is very youn |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
