RPMI-1640 培养基

MFCD00217820

NA.75

L-glutamine: yes
HEPES: no
phenol red: yes
NaHCO₃: no
sodium pyruvate: no

洛斯维·帕克纪念研究所培养基通常被称为PRMI。 是一种用于细胞培养和组织培养的培养基。该培养基含有磷酸，适用于含有5%二氧化碳的气体。它可用于人淋巴细胞生长。

PRMI-1640培养基可用于以下应用：

• 用于细胞培养培养基(比如RPMI1640培养基加上其他组分可以用来培养小鼠胸腺瘤EL-4)
• 用于脾细胞分枝杆菌感染(在无抗生素PRMI1640培养基)
• 用于细胞分离和培养
• 用于淋巴细胞分离和培养，ELISPOT分析，体外增生实验培养基(组分之一)
• gp39基因表达和CD40-免疫球蛋白结合分析培养基
• 用于制备抗真菌剂

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

按照每升培养基10.4克粉末配制。

补充2.0 g/L碳酸氢钠。

sterile

powder

cell culture | mammalian: suitable

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

ambient

2-8℃

11 - Combustible Solids

WGK 1