Monoclonal Anti-Connexin-43 antibody produced in mouse

MFCD00803532

Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.
The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes.

Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.

synthetic connexin-43 peptide (362-381).

Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in:

• fluorescent immunostaining
• western blotting
• immunocytochemistry
• preparation of immuno-affinity columns

Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells.

200

mouse

unconjugated

ascites fluid

primary antibodies

CXN-6, monoclonal

antigen 43 kDa

15 mM sodium azide

rat, human, hamster, bovine, mouse

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:8,000-1:16.000 using mouse whole brain extract

IgM

P17302

dry ice

−20℃

human ... GJA1(2697), GJA1P1(2698)
mouse ... Gja1(14609)
rat ... Gja1(24392)

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13 - Non Combustible Solids

WGK 1