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产品性质
| biological source【生物来源】 | human placenta |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | modal no. 71 |
| morphology【形态学】 | Epithelial |
| products【产品】 | Human chorionic gonadotrophin (hCG), somatomammotrophin |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human choriocarcinoma |
| Cell Line Description【细胞系描述】 | One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo . |
| Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin, 5% CO2; 37℃. Vacuolisation will occur at confluency. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
