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产品性质
biological source【生物来源】 | human blood |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | Not specified |
morphology【形态学】 | Lymphoblast |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human B cell lymphocyte, EBV transformed |
Cell Line Description【细胞系描述】 | Peripheral blood lymphocytes from a male Tunisian, immortalised by EBV transformation. The cell line contains the following deletions, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4. |
Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |