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EZviewTM Red 抗-HA 亲和凝胶
EZviewTM Red Anti-HA Affinity Gel
产品别名
EZviewTM Red 抗-HA 亲和凝胶
EZviewTM Red Anti-HA Affinity Gel
Anti-HA
抗-HA
产品性质
shelf life【保质期】 | 1 yr at 2‑8 ℃ |
Quality Level【质量水平】 | 200 |
analyte chemical class(es) | proteins (hemagglutinin) |
technique(s) | affinity chromatography: suitable immunoprecipitation (IP): suitable |
matrix【基质】 | crosslinked agarose |
capacity【容量】 | ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein) |
storage temp.【储存温度】 | 2-8℃ |
基本信息
General description【一般描述】 | EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition. |
Specificity【特异性】 | 结合特异性:HA-标签多肽(N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C)。它可以识别HA标签蛋白的还原形式,并与N-端或C-端HA标签融合蛋白反应。 |
Application【应用】 | EZview™ Red Anti-HA亲和凝胶已用于免疫印迹分析和免疫沉淀测定。 适用于亲和捕获(免疫沉淀) 大肠杆菌 或哺乳动物细胞中N-端或C-端带HA标记融合蛋白。 当进行小规模的亲和捕获时,如免疫沉淀,在微离心管中很难看到亲和基质。 偶然吸入树脂会导致定量结果变化。The EZview™ Red 亲和凝胶大大降低了颗粒损失的风险。 EZview树脂的性能和传统的非彩色亲和凝胶一样好,然而它使得用户很容易地从上清中辨别颗粒。 这与更准确的数据关联,因为蛋白质损失更少。 |
Features and Benefits【特点和优势】 |
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Other Notes【其他说明】 | 亲和凝胶含有亲和纯化的抗HA小鼠单克隆抗体(克隆HA-7),共价连接于珠状琼脂糖上。 HA标签与人流感病毒血凝素(HA)的98-106氨基酸残基(YPYDVPDYA)相对应。 |
Physical form【外形】 | 1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中 |
Legal Information【法律信息】 | EZview is a trademark of sigma-aldrich Co. LLC |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |